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. 2021 Nov 18;24(6):1025–1035. doi: 10.1093/europace/euab273

Figure 4.

Figure 4

Post-translational regulation of Ca2+ handling proteins. Whole cell protein extracts, from the hearts of mice collected 2 h after last pacing or sham intervention, were investigated for expression of total of phosphorylated proteins involved in Ca2+ handling. (A). Representative western blots of NCX; SERC 2A; RyR2 phosphorylated at serine residue 2814, 2030, 2808, and total (t); CaMKII phosphorylated at threonine residue 286 and total (t); PLN phosphorylated at serine residue 16 and threonine residue 17 as well as total (t); and GAPDH. (B). Summary statistics for phosphorylated protein with statistically different expression in paced vs. sham hearts (labelled with orange background on A). Results expressed as individual data points with mean ± standard deviation: *P ≤ 0.05 vs. sham. CaMKII, calcium/calmodulin-dependent protein kinase II; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NCX, sodium-calcium exchanger; PLN, phospholamban; RyR2, Ryanodine receptor 2; SERC 2A, sarcoplasmic reticulum Ca2+-ATPase.