Figure 6. PIB-KD colonized mice but did not induce constipation.
1 × 109 CFU PIB-KD or PIB-WT (0.1 mL) was administered orally to each mouse. (A) Typical immunochemistry section of mucus layers of colons from different groups of mice. (B) Quantification of mucus layer thickness (n = 6). (C) Representative immunochemistry results for colonic ganglia. (D and E) Quantification of ganglia and neurons (n = 9). (F) PIB2013 detection of fecal PIB-WT and PIB-KD in the stools. (G) Number of stools within 2 hours (n = 10). (H) Fecal water (n = 10, except PIB-WT n = 9). (I) GTT time (n = 10). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 (1-way ANOVA Tukey’s multiple-comparison test). Scale bars: 200 μm.