Figure 7. CLSTN2 and PTPRO are predictive of mDA neuron differentiation and can give rise to highly enriched mDA neurons after progenitor sorting and transplantation.

(A) Diagram of surface marker reporter cell lines and experimental schematics for in vitro and in vivo maturation. (B and C) Neurospheres matured in vitro and (B) immunostained for TH and (C) statistical analysis (n = 3 batches with 5 neurospheres per batch). Scale bars: 25 μm. ***P < 0.001, by multiple unpaired t test with Holm-Šidák correction. (D) Correlation between the surface marker progenitor ratio and the TH⁺ neuron ratio. See Figure 9A for a diagram of the cell lines used. (E) Unsorted progenitor-, CLSTN2⁺ progenitor–, and PTPRO⁺ progenitor–derived grafts immunostained for human nuclei (hN) and TH. Scale bars: 100 μm and 20 μm (for the enlarged insets [i] and [ii], which represent the edge and center area of the graft, respectively). (F) Quantification of the TH⁺ neurons ratio in grafts. n = 6 (unsorted), n = 5 (CLSTN2), and n = 7 (PTPRO). **P < 0.01 and ***P < 0.001, by 1-way ANOVA followed by Tukey’s multiple-comparison test. Sort, sorted; unsort, unsorted.