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. 2022 Jul 15;132(14):e158524. doi: 10.1172/JCI158524

Figure 7. Extended ex vivo injury selectively targeting glial membranes results in secondary axonal degeneration.

Figure 7

Triangularis sterni nerve–muscle preparations from Glial (A) and WT (B) mice were treated ex vivo with anti-GM1 Ab or anti-sulfatide Ab, respectively, and a source of complement (injury, Inj) or Ab alone (control, Con) for 20 hours. (A) Anti-GM1 Ab (orange) and complement (green) deposition along the distal motor nerve was strongly enriched at the paranodes (arrowheads) in injured compared with control tissue. Loss of axonal integrity along the distal nerve was monitored by presence of neurofilament H immunostaining (NFH, magenta) and cytosolic CFP (blue). (B) The experiment was repeated in WT mice using an anti-sulfatide Ab; the results reflect those reported in A. Scale bars: 10 μm (A) and 20 μm (B). Results are represented as the mean ± SEM. n = 3/treatment: 25–54 NoRs/mouse (median = 39) were analyzed. **P < 0.01, ***P < 0.001 compared with control by 1-tailed Student’s t test.