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. 2022 Jul 15;132(14):e154333. doi: 10.1172/JCI154333

Figure 1. FoxO1 expression is upregulated in hepatic and adipose tissue macrophages in dietary obese mice.

Figure 1

C57BL/6 mice (male, 8 weeks old) were fed RC and HFD for 8 weeks, followed by the isolation of hepatic macrophages from the liver and adipose stromal vascular cells (SVCs) from epididymal adipose tissues. Total RNAs were prepared from hepatic macrophages and SVCs and analyzed by real-time qRT-PCR. (A) Body weight. (B) Fasting blood glucose levels. (C) Fasting plasma insulin levels. (D) HOMA-IR. (E) Hepatic macrophage mRNA levels. (F) Hepatic macrophage TNF-α mRNA levels. (G) Adipose SVC mRNA levels. (H) Adipose SVC TNF-α mRNA levels. Fasting blood glucose and plasma insulin levels were determined after 16-hour fasting. Data are expressed as mean ± SEM (n = 6). Statistical analysis in A–D, F, and H was performed using a 2-tailed, unpaired t test, and in E and G using a 1-tailed, unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001.