Figure 4. Myeloid FoxO1 depletion improves energy expenditure and ameliorates steatosis in dietary obese mice.

MøFoxO1-KO and WT littermates (male, 8 weeks old) were fed an HFD for 34 weeks, followed by the determination of energy expenditure. (A) Respiratory exchange ratio (RER) in dark and light cycles. (B) VO₂ consumption rates in dark and light cycles. (C) Oil Red O staining of liver sections of WT mice (original magnification, ×20). Scale bar: 50 μm. (D) Oil Red O staining of liver sections of MøFoxO1-KO mice (original magnification, ×20). Scale bar: 50 μm. (E) Hepatic TG content. (F) Hepatic expression of key lipogenic genes, as determined by real-time qRT-PCR assay. (G) Hepatic expression of key genes in fatty acid oxidation. (H) Hepatic expression of genes in VLDL-TG production. Data are expressed as mean ± SEM (n = 7–9). Statistical analysis in A, B, and H was performed using a 1-tailed, unpaired t test, and in E–G using a 2-tailed, unpaired t test. *P < 0.05, **P < 0.01.