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The p21 gene is the direct target of miR-128-5p. (a) The predicted miR-128-5p binding sequences in the p21 gene and the designed mut sequence (p21-mut). (b) The miR-128-5p mimic was cotransfected with the wild-type or mutated p21 3′UTR sequence vectors in HEK 293T cells. The relative firefly luciferase activity was calculated. Three independent experiments were repeated. ∗P < 0.05 versus the respective NC.
