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. 2022 Jul 7;2022:5498743. doi: 10.1155/2022/5498743

Figure 1.

Figure 1

SIRT4 expression is downregulated in PCa. (a) The results of HE staining. (b) SIRT4 protein expression was detected by immunohistochemistry in paired of PCa tissue and adjacent tissue. (c) SIRT4 protein expression was analyzed by western blotting in 24 pairs of PCa tissues and prostate normal tissues. The results were measured by grayscale analysis. (d, e) The qRT-PCR and western blotting were used to detect the SIRT4 expression in n a normal prostate epithelial cell line (RWPE-1), and prostate cancer cell lines (DU145, PC-3, PC-3M, 22rv1, and LNCap), respectively, magnification ×100, scale bar = 100 μm, magnification ×400, scale bar = 25 μm ∗P < 0.05∗∗P < 0.01, ∗∗∗P < 0.001.