Fig. 5.

Isolated A-IDE-KO islets show constitutive glucagon and insulin secretion. (a) Glucagon secreted by islets, isolated from control and A-IDE-KO mice, in response to low and high glucose (1 mmol/l glucose; 16 mmol/l glucose). (b) Glucagon secreted by isolated islets in response to insulin inhibition (in the presence of 1 mmol/l glucose). (c) Glucagon secreted by wild-type C57Bl/6J mouse islets treated with 10 μmol/l 6bK (selective IDE inhibitor) for 30 min, in response to low and high glucose. (d) Insulin secreted by isolated islets in response to low and high glucose. n = 17–21 islet batches. Data are presented as means ± SEM. *p<0.05 vs control 1 mmol/l glucose; ^†††p<0.001 vs control 16 mmol/l glucose; ^‡p<0.05 vs A-IDE-KO 16 mmol/l glucose. G, mmol/l glucose; INS, insulin