Figure 1.

Expression of E5 genes in human foreskin keratincutes (HFKs). (A) RT‐PCR. Specific primer sets for 16E5 and 6bE5 were used for RT‐PCR assays on RNA samples from three biological replicates, GAPDH was used as internal control. LXSN was vector control. (B) Immunuprecipitation and western blot (IP/WB). RIPA lysates from E5 transduced HFKs were immunoprecipitated and blotted with monoclonal antibody against AU1. LXSN was vector control. RT‐PCR, reverse‐transcription polymerase chain reaction