Figure 2.

Construction of the HPV‐16 E5 mutant, H77YA78I, 16HA (A) Alignment of low‐risk (yellow) and high‐risk (purple) HPV E5 protein sequences. Mutations were made in boxed region. Amino acids histidine and alanine (highly conserved in high‐risk HPVs) were swapped for tyrosine and isoleucine (highly conserved in low‐risk HPVs). (B) Mutant co‐localization with the ER‐marker calnexin (green) in stably transduced HECs and transfected COS‐1 cells. AU1‐tagged E5 is visualized by an anti‐AU1 antibody (red). DAPI (blue) is the nuclear stain. DAPI, 4′,6‐diamidino‐2‐phenylindole; HPV, human papillomavirus