Figure 3.

Real‐time RT‐PCR confirmation of genes in 16E5 arrays. Four genes altered by 16E5 in the microarray analysis were chosen for confirmation by real‐time RT‐PCR (A–D). 6bE5 did not cause significant changes in expression of these genes. In addition, 16E5 mutant 16HA is also defective for a reduction in these genes. Three biological replicates (BR) were tested for each gene. Data for all experiments are normalized to GAPDH. n = 3. Bars represent means ± SEM. *Indicates p value < 0.05 as determined by a paired student's t test. RT‐PCR, reverse‐transcription polymerase chain reaction; SEM, standard error of mean