Table 1.
Microarray analysis reveals 16E5‐induced alteration of the keratinocyte gene expression profile.
| (A) Less than 25 genes were found to be consistently changed (>1.5 fold in each array, p < 0.01) in 16E5‐expressing cells as compared to LXSN‐expressing cells. | |||
|---|---|---|---|
| Primary sequence name | Accession # | p Value | Fold change |
| AHNAK | NM_001620 | 0.00 | −1.65 |
| FLJ00399 | AK090477 | 0.01 | −1.70 |
| GPR135 | NM_022571 | 0.00 | −1.71 |
| FLJ20802 | AK000809 | 0.00 | −1.71 |
| SPRED1 | NM_152594 | 0.00 | −1.73 |
| NR3C1 | U25029 | 0.00 | −1.76 |
| LOC440345 | AK123481 | 0.00 | −1.76 |
| LOC440248 | NM_199045 | 0.00 | −1.80 |
| BX090412 | BX090412 | 0.00 | −1.90 |
| APP | CK8188527 | 0.00 | −1.94 |
| PRO1073 | AF001542 | 0.00 | −1.95 |
| PTGS2 | NM_000963 | 0.00 | −1.99 |
| PITPNC1 | AK094724 | 0.00 | −2.03 |
| LOC440345 | AK123481 | 0.00 | −2.08 |
| ADH1A | BX647987 | 0.00 | −2.08 |
| CYP3A7 | NM_000765 | 0.00 | −2.18 |
| PCDH9 | BC008476 | 0.01 | −2.28 |
| LOC283970 | XM_934220 | 0.00 | −2.60 |
| UBE2E1 | NM_003341 | 0.00 | −2.73 |
| LOC150759 | AK057596 | 0.00 | −3.14 |
| CD47 | NM_001777 | 0.00 | −3.20 |
| MED26 | BC030138 | 0.00 | −6.11 |
| (B) Genes were classified according to enriched gene ontology terms. | |||
|---|---|---|---|
| GO Class ID | Definitions | Counts | Fractions |
| GO:0008152 | metabolism | 6 | 28.60% |
| GO:0006629 | lipid metabolism | 6 | 28.60% |
| GO:0009058 | biosynthesis | 3 | 14.30% |
| GO:0007275 | development | 3 | 14.30% |
| GO:0007165 | signal transduction | 1 | 4.80% |
| GO:0007154 | cell communication | 1 | 4.80% |
| GO:0040007 | growth | 1 | 4.80% |
| Total | 21 | 100.00% | |
Note: Microarray was performed on three different donor pools of primary foreskin keratinocytes which were stably transduced with 16E5 or LXSN. Dye swap was performed for each replicate. (A) Less than 25 genes were found to be consistently changed (>1.5 fold in each array, p < 0.01) in 16E5‐expressing cells as compared to LXSN‐expressing cells. (B) Genes were classified according to enriched gene ontology terms. Significantly represented (p < 0.01) biological processes are shown.