Figure 6.

Clusterin regulates ECM remodeling by MMP activation. Serum‐starved HTM cells transduced with AdCLU compared to AdMT shows – (a) no change in MMP2 mRNA expression, (b) no change on MMP2 protein expression. GAPDH was used as a loading control. (c) significant decrease in mRNA expression of tissue inhibitors of metalloproteinases (TIMP1 & TIMP2). (d) and (e) significant increase in active form of MMP2 (62 kDa) as visualized by gelatin zymography. No significant change is seen in pro‐MMP2 (72 kDa). MMP1, and MMP‐9 were detected. Values represent the mean ± SEM, where n = 3–4 (biological replicates). *p ≤ 0.05 was considered significant. AdCLU, adenovirus‐mediated constitutive expression of clusterin; ECM, extracellular matrix; mRNA, messenger RNA; SEM, standard error of mean; WCL, whole‐cell lysate