Figure 7.

Clusterin supplementation attenuates TGFβ2‐induced fibrogenic process. Serum‐starved HTM cells treated with TGFβ2 exhibited significant increase in COL1A (WCL), TSP1 (WCL and CM), and PAI1 (CM) which was significantly suppressed by supplementation of rhCLU. β‐actin or GAPDH was used as a loading control for WCL, and ponceau‐S band was used as a loading control for CM. Values represent the mean ± SEM, where n = 4 (biological replicates). * p ≤ 0.05 was considered significant. CM, conditioned media; SEM, standard error of mean; WCL, whole‐cell lysate