Comparison of amino acid sequences of the GyrB proteins of B. hyodysenteriae (BhyoGyrB), B. burgdorferi (BbGyrB), and E. coli (EcoGyrB) and the ParE protein of E. coli (EcoParE). B. hyodysenteriae GyrB exhibits overall sequence identities of 56, 42, and 37% with B. burgdorferi GyrB, E. coli GyrB, and E. coli ParE, respectively. In regions A to D, the B. hyodysenteriae GyrB protein exhibits the following sequence identities with B. burgdorferi GyrB, E. coli GyrB, and E. coli ParE, respectively: region A, 44, 48, and 22%; region B, 50, 58, and 34%; region C, 60, 54, and 33%; and region D, (D) 58, 60, and 21%. B. hyodysenteriae Cnr strains isolated in this study have amino acid mutations at positions Gly78 and Thr166, (indicated by asterisks).