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Genotype analyses of antibiotic resistance determinants of strains SH (Kmr Cmr), 435A (Cnr), and QM-1 (Cnr Kmr Cmr). Six other triply resistant strains (QM-2 to QM-7) were examined and gave results identical to those obtained with strain QM-1. (A) PCR amplification to detect the kan gene (lanes 1, 3, and 5) and the cat gene (lanes 2, 4, and 6). (B) DNA sequence analysis to detect the gyrB nucleotide change (corresponding amino acid change) associated with coumermycin resistance.
