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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nitric Oxide. 2022 Jun 3;125-126:12–22. doi: 10.1016/j.niox.2022.06.001

Figure 3. Oleate binding to wild type Cytoglobin and distal histidine (His81) mutants determined by differential spectroscopy.

Figure 3.

Cytoglobins (5–10 μM) were mixed with increasing concentrations of oleic acid. Left panels indicate the absorbance changes upon addition of increasing amounts of oleate. The concentrations of oleate are indicated in each panel. Middle panels show the difference spectra -subtracting the initial spectrum from each spectrum. Right panels show the fit to determine the apparent KD values by fitting the absorbance differences at the wavelengths showing the largest absorbance change versus the concentration of oleate. The red line denotes the fit of the data to Equation 1. The 500nm-700nm range in the left and middle panels is enlarged to show the spectral changes in detail.