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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Cell Host Microbe. 2022 May 30;30(7):988–1002.e6. doi: 10.1016/j.chom.2022.05.004

Figure 1. An Allobaculum species from an ulcerative colitis patient exacerbates acute and chronic colitis in gnotobiotic mice.

Figure 1.

(A) Identification and Isolation of IgA-coated Allobaculum sp. 128 from an ulcerative colitis patient. (B) Scanning electron micrographs of Allobaculum sp. 128 in vitro. Scale bars, 2μm (top), 10μm (bottom). (C-H) Germ-free WT mice were gavaged and colonized for seven days before treatment with 2% DSS-H2O ad libitum. (C) Fecal microbiota on d0 (first bar) and d7 (bars 2–5) of DSS. (D-E) Colons on d7 and representative H&E sections. Scale bars, 1mm. (F) Colon length on d7. (G) Fecal lipocalin (LCN2) on d2. (H) Histopathology scores. (I-L) Acute DSS colitis in Rag1−/− gnotobiotic mice: colon length (I), d2 fecal lipocalin (J), lamina propria CD45+Ly6G+ neutrophils (K), and colon explant cytokines (L). (M-N) Spontaneous colitis in Il10−/− gnotobiotic mice: fecal LCN2 (M) and colon histopathology scores (N). Welch’s t-test was used to compare microbiota groups. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. Error bars show mean ± SEM. (C-H) show one of N=6 independent experiments, n=5–6 mice per group. (I-L) show one of N=4 independent experiments, n=4–9 mice/group. (M-N) show one of N=3 independent experiments, n=5 mice/group.