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. Author manuscript; available in PMC: 2023 Jul 11.
Published in final edited form as: Curr Biol. 2022 May 27;32(13):2962–2971.e4. doi: 10.1016/j.cub.2022.05.009

Figure 3. The 359bp satellite deletion rescues mh[sim] genome integrity and fertility.

Figure 3.

(A) The Zhr X chromosome lacks the 11Mb pericentromeric 359bp satellite array. (B) A 10-cycle PCR distinguishes between wildtype 359bp copy number and the 359bp deletion (Zhr) and validates the recombined mh[mel],Zhr and recombined mh[sim],Zhr X chromosomes. (C) γH2Av signal in mh[mel],Zhr and mh[sim],Zhr ovaries and the quantification of normalized fluorescent signal intensity. Dotted lines correspond to mh[mel] and mh[sim] averages reported in Figure 2D. (D) Ovary size of mh[mel],Zhr and mh[sim],Zhr females. (E) Number of mature eggs per ovary pair from mh[mel],Zhr and mh[sim],Zhr females. (F) Progeny counts from mh[mel],Zhr and mh[sim],Zhr females crossed to wildtype (w1118) males. In panels D, E, and F, dotted lines correspond to mh[mel] and mh[sim] averages reported in Figure 1E, 1F, and 1C, respectively. (t-test: “n.s.” p > 0.05, scale bar = 25μm)