Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Monoclonal anti-FLAG M2 | Sigma-Aldrich | Cat#F3165 |
| Mouse Monoclonal 12G10 anti-αTubulin | Developmental Studies Hybridoma Bank | Cat#12G10 anti-alpha-tubulin, RRID:AB_1157911 |
| Mouse Monocolonal anti-Histone 2A Gamma Variant, Phosphorylated | Developmental Studies Hybridoma Bank | Cat#UNC93–5.2.1, RRID:AB_261807 |
| Chicken Polyconal anti-Green Fluorescent Protein | Aves Labs | Cat# GFP-1010, RRID:AB_230731 |
| Chemicals, peptides, and recombinant proteins | ||
| Phusion High-Fidelity DNA Polymerase | NEB | Cat#M0530 |
| RIPA buffer | Cell Signaling Technology | Cat#9806 |
| Protease Inhibitor Cocktail | Roche | Cat#11873580001 |
| PMSF | Cell Signaling Technology | Cat#8553 |
| Benzonase | Sigma-Aldrich | Cat#E1014 |
| ProLong Gold Antifade Mountant with DAPI | ThermoFisher Scientific | Cat#P36931 |
| Critical commercial assays | ||
| APAgene GOLD Genome Walking Kit | Bio S&T, Inc | BT901-RT |
| Kwikquant Western Blot Detection Kit | Kindle Biosciences | Cat#R1004 |
| Deposited data | ||
| mh alleles from D. simulans | This manuscript | OL546458..64 |
| Top2 alleles from D. simulans | This manuscript | OL156853..59 |
| Experimental models: Organisms/strains | ||
| yw; nos-Cas9(II-attP40) | Best Gene, Inc | N/A |
| yw; PBac[y + -attP-9A]VK00018 | Bloomington Drosophila Stock Center | BDSC:9736 |
| Gal4::VP16-nos | Bloomington Drosophila Stock Center | BDSC:64277 |
| Zhr 1 | Bloomington Drosophila Stock Center | BDSC:25140 |
| mnk l6 | N. Phadnis | N/A |
| Top2 17−6 | P. Geyer | N/A |
| w 1118 | MTL laboratory | N/A |
| mh 1 | Bloomington Drosophila Stock Center | BDSC:7130 |
| P{gcid.EGFP.cidIII}.2 | K. McKim, Schuh et al.75 | N/A |
| Oligonucleotides | ||
| Primers to amplify and sequence mh alleles from D. simulans, see Table S3 | This manuscript | N/A |
| Primers to screen mh CRISPR transformants, see Table S3 | This manuscript | N/A |
| Primers to screen for PCR-introduced mutations in the cloned mh UAS constructs, see Table S3 | This manuscript | N/A |
| Primers to detect native mh locus, see Table S3 | This manuscript | N/A |
| Primers to screen for 359-bp deletion (Zhr), see Table S3 | Rosic et al.9 | N/A |
| Primers for Genome Walking, see Table S3 | This manuscript | N/A |
| Primers to amplify and sequence Top2 alleles from D. simulans, see Table S3 | This manuscript | N/A |
| Recombinant DNA | ||
| Plasmid: pBFv-U6.2 | Addgene | Addgene#138400 |
| Plasmid: pBFv-U6.2B | Addgene | Addgene#138401 |
| Plasmid: 3xFLAG:mh[mel] and 3xFLAG:mh[sim] HDR plasmids | This manuscript | N/A |
| Plasmid: pUASp-attB | Drosophila Genomics Resource Center | DGRC#1358 |
| Software and algorithms | ||
| Geneious v 11.1.5 | Biomatters | https://www.geneious.com/ |
| FIJI | ImageJ2 | https://imagej.net/software/fiji/ |