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. Author manuscript; available in PMC: 2023 Jul 11.
Published in final edited form as: Curr Biol. 2022 May 27;32(13):2948–2955.e4. doi: 10.1016/j.cub.2022.05.007
Reagent or Resource Source Identifier
Deposited data
DNA and RNA sequences This Paper https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA666913
VEuPathDB VeuPathDB https://veupathdb.org/veupathdb/app
Aniseed aniseed https://www.aniseed.cnrs.fr/aniseed/species/
Busco v3 Swiss Institute of Bioinformatics https://busco.ezlab.org/
Interproscan v71.0 InterPro https://www.ebi.ac.uk/interpro/about/interproscan/
KEGG Kanehisa Laboratories https://www.kegg.jp/
Software and algorithms
Trimmomatic v0.3 USADELL Lab http://www.usadellab.org/cms/?page=trimmomatic
SPAdes v3.13. Saint Petersburg State University https://github.com/ablab/spades
PBSuite v15.8.2 Pacific Biosciences https://sourceforge.net/projects/pb-jelly/
Canu v1.6 Maryland Bioinformatics Labs https://github.com/marbl/canu/releases/tag/v2.2
ABySS v2.02 BC Cancer Canada’s Michael Smith Genome Sciences Centre https://github.com/bcgsc/abyss
Bowtie2 v1.2.2 Johns Hopkins University http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Bedtools University of Utah https://bedtools.readthedocs.io/en/latest/index.html
VizBin university du luxembourg https://claczny.github.io/VizBin/
CAT Universiteit Utrecht https://github.com/dutilh/CAT
Trinity/Trinotate pipeline v2.4.0 The Broad Institute https://github.com/trinityrnaseq/trinityrnaseq
BLASTp National Center for Biotechnology Information ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/
Transdecoder The Broad Institute https://github.com/TransDecoder/TransDecoder
BBmap Brian Bushnell https://sourceforge.net/projects/bbmap/
CDhit v4.6.8 UCSD https://sourceforge.net/projects/cdhit/
Prokka The University of Melbourne https://github.com/tseemann/prokka
Maker University of Utah http://www.yandell-lab.org/software/maker.html
R and bash code This paper https://github.com/cpaight/Nephromyces
Critical commercial assays
MiSeq Reagent Kit v3 Illumina Product Number MS-102–3003
HiSeq 2500 2×250 HiSeq SBS v4 Illumina Product Number FC-401–4003
SMRT cells Pacific Biosciences Product Number 102-281-700
Zymo Quick-RNA kit Zymo Catalogue number R1054
Illumina TruSeq RNA kit Illumina Product Number RS-122–2001
Prolong Gold with DAPI ThermoFisher Product Number P-36931
Experimental models: Organisms/strains
M. manhattensis This paper Live collection, Greenwich Bay, RI (41.653N, −71.452W)
M. occidentalis This paper Live collection by Gulf Specimens Marine Laboratories, Inc. from Alligator Harbor, FL (29.899N, −84.381W)
Isochrysis galbana National Center for Marine Algae and Microbiota CCMP1323
Chaetoceras sp. National Center for Marine Algae and Microbiota CCMP209