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. 2022 Apr 21;47(9):1710–1720. doi: 10.1038/s41386-022-01323-3

Fig. 1. Downregulation of klotho in the NAc contributes to the behavioral responses to stress in mice.

Fig. 1

a Representative images of western blotting and histograms showing the level of klotho protein in whole brains from the control, susceptible and unsusceptible mice (n = 5 mice per group). b The expression of klotho protein in the PFC, Hip, and NAc from the control, susceptible and unsusceptible mice (n = 5 mice per group). c Timeline of the experimental procedures. d Fluorescence image of a fixed brain section which expressed AAV-KL-KD in the NAc 14 days after stereotactic injection (Scale bar = 2000 μm). e Representative western blotting images and histograms showing the protein expression of klotho in the NAc in each group (n = 6 mice per group). f KL-KD in the NAc significantly decreased sucrose preference in mice. g, h KL-KD in the NAc increased the immobility time in FST (g) and TST (h) in mice. i Representative images of movement tracks in the OFT. j KL-KD in the NAc did not affect total traveled distance (left), but significantly decreased the time spent in the central area (right) in the OFT in mice. For (a), (b) and (e), data are presented as normalized mean ± SEM. For (f–j), all box and whisker plot displays the median, first and third quartiles (boxes), and the min-max (whiskers) (n = 10 mice per group). *p < 0.05 and **p < 0.01 vs. control. #p < 0.05 and ##p < 0.01 vs. susceptible group or GFP group.