Skip to main content
. 2022 Apr 21;47(9):1710–1720. doi: 10.1038/s41386-022-01323-3

Fig. 3. Modulation of klotho level in the NAc regulates the total and surface expression of GluN2B in both the naive and susceptible mice.

Fig. 3

a Knockdown of accumbal klotho did not affect the total GluN1 and GluN2A protein levels, while it significantly decreased the total GluN2B and PSD-95 protein expression in the NAc (n = 4 mice per group; **p < 0.01 vs. control). b1-2 Western blotting analysis revealed that genetic knockdown of accumbal klotho produced no effect on the expressions of GluN1 (b1) and GluN2A (b2) in both the surface and intracellular pools in the NAc of mice. (b3) Genetic knockdown of accumbal klotho significantly reduced GluN2B expression in the surface pool but not in the intracellular pool (n = 4 mice per group; **p < 0.01 vs. control). c Representative immunoblots showing the total protein expressions of GluN1, GluN2A, GluN2B and PSD-95 in the NAc of mice from each group. Statistical results show that CSDS decreased the protein expressions of GluN2B and PSD-95 in NAc, while klotho overexpression reversed the effects of CSDS (n = 6 mice per group; **p < 0.01 vs. control; ##p < 0.01 vs. GFP group or susceptible group). d1-3 Representative immunoblots showing the expressions of GluN1 (d1), GluN2A (d2) and GluN2B (d3) in both the surface and intracellular pool in NAc. Statistical results show that CSDS caused a reduction of GluN2B in the surface pool of NAc, while klotho overexpression reversed the decline of surface GluN2B expression (n = 6 mice per group; **p < 0.01 vs. control; ##p < 0.01 vs. GFP group or susceptible group). All data were presented as normalized mean ± SEM.