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. 2022 Jul 14;7:79. doi: 10.1038/s41541-022-00500-1

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Diagram for generating ferret antisera against the NA antigens (N1-GD19, N2-HK19e, and N2-HK19c) from the recommended egg and cell-based IAV vaccine strains for the 2020–21 northern hemisphere season. Ferret sera were collected 21 days post intranasal inoculation with PR8 double gene reassortant viruses carrying H6 and one of the vaccine strain NAs. b Specificity and reactivity of each ferret antiserum was assessed by the ELLA using PR8 single gene reassortant viruses that carry the IAV vaccine strain NAs. Representative data from a single analysis performed in duplicate are displayed. Source data are provided in the source data file.