Figure 3.

Beneficial effects of AMO administration against miR-33b to miR-33b KI mice for 6 weeks on CaCl₂-induced AAA formation. (a) Copy number changes of miR-33a/b in abdominal aorta over a 6-week period, n = 8 mice in each group. One-way ANOVA with Dunnett’s multiple comparison test. ***P < 0.001 compared with baseline analysis. (b) Protocol scheme of administration of AMOs for 6 weeks. (c) Serial body weight changes during the experimental period, n = 20 mice with control AmNA administration, n = 19 mice with anti-miR-33a administration, and n = 28 mice with anti-miR-33b administration. (d) Representative photographs of CaCl₂-induced AAA with administration of the indicated AMOs to miR-33b KI mice once a week for 6 weeks. White bars indicate 1 mm. (e) Maximum diameter and lesion length of abdominal aorta between left renal artery and terminal aorta of CaCl₂-induced AAA, n = 10 mice in each group. Mann–Whitney test (left) and unpaired two-tailed t test (right). ***P < 0.001. (f) Expression levels of miR-33a (left) and miR-33b (mid), and absolute copy numbers of miR-33a and miR-33b (right) in the CaCl₂-induced AAA of miR-33b KI mice with administration of the indicated AMOs, n = 5 mice in each group. Unpaired two-tailed t test (left and right) and Mann–Whitney test (mid). *P < 0.05 and **P < 0.01. ns is defined as not significant. All data represent mean ± SEM.