Table 1.
Characterization of five duplex PCR reactions in sterlet.
| Groups | GenBank accession no |
Primer sequences (5′ − 3′) | Annealing temperature (℃) | Repeat motif(s) | Length | Chromosomal Location |
|---|---|---|---|---|---|---|
| A | OM320532 | F:GCGTTCACTGAGTCAATGCA | 60 | (TATC)14 | 222 | 28 |
| R:CTGGACAGAGAACAGATAGCGT | ||||||
| OM320533 | F:ACCTGCCTTCTTCCAGCTTT | (AGAT)12 | 299 | 3 | ||
| R:AATCACGGACAGCCAAGAGG | ||||||
| B | OM320534 | F:ACAGTGGACAATGTGGCTCA | 60 | (TTTA)10 | 256 | 9 |
| R:CCAGGACCACGGCTAGTTTT | ||||||
| OM320535 | F:TCACGTTGATCAGGGTCTTCA | (ATAG)12 | 181 | 9 | ||
| R:TCCACAAACACAAAACATTTGCT | ||||||
| C | OM320536 | F:ACCCTCCCACAGATGCTGTA | 60 | (TTTA)8 | 118 | 13 |
| R:TGATAGTTCAAATTGACGAAGGCT | ||||||
| OM320537 | F:TCGGGGGTAAAATAATGGGAGA | (AATA)9 | 155 | Unknow | ||
| R:CTAACTCGGCCCCAAACCAT | ||||||
| D | OM320538 | F:GGCCACCACTGTTGATCTGA | 60 | (AAAT)9 | 229 | 16 |
| R:GCCATTCTCCTCCCTGACAC | ||||||
| OM320539 | F:TGTGCTTACTTGCATCTGTTGT | (CTAT)9 | 303 | 23 | ||
| R:CGCTCCGCTCTAAGAAGACA | ||||||
| E | OM320540 | F:GGACATTCTCATTCTCAGCTGC | 60 | (ATAC)9 | 239 | 3 |
| R:ATCACGATCCATGCCTTGCA | ||||||
| OM320541 | F:GCATGTGTCACTGAGATAGTTGC | (TATT)9 | 191 | 1 | ||
| R:TGCTGCAGTTGAGGTCCATT |