Transient transfection and purification of SARS-CoV-2 spike protein from mammalian cells

. 2022 Jul 15;3(3):101603. doi: 10.1016/j.xpro.2022.101603

Other Solutions

Name	Reagents
1× Buffer W	Dilute 100 mL of 10× Buffer W in 900 mL of Milli-Q ddH₂O. Store at 4°C for up to 12 months. No filtering or degassed water required if using Milli-Q double-distilled water.
1× Buffer E	Dilute 25 mL of 10× Buffer E in 225 mL of Milli-Q ddH₂O. Store at 4°C for up to 12 months. No filtering or degassed water required if using Milli-Q double-distilled water.
1× Buffer R	Dilute 100 mL of 10× Buffer R in 900 mL of Milli-Q ddH₂O. Store at 4°C for up to 12 months. No filtering or degassed water required if using Milli-Q double-distilled water.
1× NuPAGE MES SDS Running Buffer	Dilute 50 mL of 20× NuPAGE MES SDS Running Buffer in 950 mL of Di-water. This buffer can be stored at room temperature (20°C–23°C) for at least 2 months provided no precipitate or microbial contamination are visible.
1 M DTT	Dissolve 154.25 mg of DTT in 1 mL of ddH₂O. Store at 4°C for up to 1 month.