REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
FreeStyle 293 Expression Medium	Gibco	12338018
Hyclone SFM4HEK293	Cytiva	SH30521.02
Opti-MEM I	Gibco	31985-070
Turbo293	SPEED Biosystems	PXX1002
10× Buffer E	IBA	2-1000
10× Buffer R	IBA	2-1002
10× Buffer W	IBA	2-1003
Strep-Tactin resin	IBA	2-1201
Sodium Azide	Sigma	S8032
BUFFER TRIS 1 M PH 8.0 BIOTECH GRD	VWR	97062-674
NaCl Solution, 5 M	Santa Cruz Biotechnology, Inc.	sc-295833
TK51768015T DTT DL-Dithiothreitol Cleland’s reagent	WW Grainger, Inc.	31FX81
Critical commercial assays
Superose 6 Increase 10/300 GL	Cytiva	29091596
NuPage 4%–12% Bis-Tris gel	Invitrogen	NP0321
NuPAGE MES SDS Running Buffer (20×)	Novex	NP0002
SimplyBlue SafeStain	Novex	LC6065
Capillaries (200 count) Tycho NT.6	NanoTemper Technologies, Inc.	TY-C001
Econo-Column® Chromatography Columns, 2.5 × 10 cm	Bio-Rad	7372512
Female luers	Bio-Rad	7318223
Silicone tubing	Bio-Rad	7318212
Amicon Ultra-15 Centrifugal Filter Unit (100 KDa cutoff)	Millipore	UFC910008
VIVAFLOW 200 PES 100 000 MWCO	Sartorius	VF20P4
Complete Filtration Unit 1 L, 0.20 μm	VWR	10040-440
4× Laemmli Sample Buffer	Bio-Rad	1610747
Precision Plus Protein Kaleidoscope	Bio-Rad	1610375
Sodium hypochlorite solution	Sigma-Aldrich	425044-250ML
Experimental models: Cell lines
Freestyle 293-F cells	Gibco	R79007
ExpiCHO-S Cells	Invitrogen	A29127
ExpiFectamine CHO Transfection Kit	Invitrogen	A29130
Recombinant DNA
pαH-S-GSAS/PP	(Wrapp et al., 2020)	N/A
pαH-S-GSAS-OMICRON	(Gobeil et al., 2022)	Addgene: 180423
pαH-S-RRAR	(Gobeil et al., 2021)	Addgene: 164569
Software and algorithms
GraphPad Prism 9.1.2	GraphPad	https://www.graphpad.com; RRID: SCR_002798
Other
Eppendorf® Centrifuge 5804/5804R	Eppendorf	EP022628146
Mini Gel Tank	Invitrogen	A25977
ChemiDoc MP Imaging System	Bio-Rad	12003154
Isotemp™ Digital Dry Baths/Block Heaters	Fisherbrand	88-860-023
Mini Rocker Rocking Platform	Bio-Rad	1660710EDU
MasterFlex Easy-Load pump	Microflex L/S	Model 07514-10
PowerPac Basic Power Supply	Bio-Rad	1645050
Tycho NT.6	NanoTemper Technologies, Inc.	N/A
NanoDrop One	Thermo Scientific	ND-ONE-W
AKTA go (FPLC)	GE Life Sciences Solutions	29383015
Fraction Collector F9-R	GE Life Sciences Solutions	29011362
1750RNR 500 μL SYR (FPLC sample syringe)	Hamilton	81265