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. 2022 Jul 1;16:100345. doi: 10.1016/j.mtbio.2022.100345

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Pathogenic microbe-infected small intestinal organoids. (A) Immunofluorescence images of human rotavirus (HRV; strains Ito)-infected (right) organoids [60]. (B) The presence of intracellular rotavirus antigen in single cells from undifferentiated and differentiated organoids analyzed by flow cytometry [60]. (C) The amount of infectious virus in undifferentiated and differentiated organoids [60]. (D) Western blot analysis of tight junction proteins (Zo-1, Occludin, Claudin-2, and Claudin-7) in Salmonella-infected organoids [67]. (E) Gentamicin protection assays of IL-22-treated Salmonella-infected organoids [65]. (F) Confocal images of organoids injected with healthy stool and C. difficile. Scale bar, 50 μm [68]. (G) Representative images of fluorescein isothiocyanate (FITC, green)-dextran leakage from the lumen of organoids treated with C. difficile toxin tcdA and tcdB [23].