FIG. 2.

Characterization of the contribution of genes on cHRIM1 to insecticidal activity. (A) Map indicating the effect of transposon insertions in genes, including insertions that did not alter insecticidal activity (active) and those that resulted in the loss of insecticidal activity (inactive). Scos, cosmid vector. (B) Map of the insert in the smallest active clone 338/2 constructed using the SalI site at the end of the chitinase gene (chi) and one transposon insertion obtained at the end of the xptA2 gene (dotted lines). (C) Map of insert in plasmid pHRIM801, where the xptA1 gene was expressed from the bacteriophage P_L promoter (P_L).