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SDS-PAGE analysis of the expression of xptA1 from the PL promoter in E. coli cells. Whole-cell extracts after 18 h of growth at 37°C. Lane 1, E. coli (pHRIM600); lane 2, E. coli. Cell fractions after growth for 4 h at 30°C (lanes 3 and 5) and for 18 h at 30°C (lanes 4 and 6). Samples were separated into inclusion bodies (lanes 3 and 4) and soluble fractions (lanes 5 and 6). Markers (M) are presented (in kilodaltons).
