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. Author manuscript; available in PMC: 2022 Jul 15.
Published in final edited form as: J Vis Exp. 2020 Sep 6;(163):10.3791/61748. doi: 10.3791/61748

Figure 5: Longitudinal section of a transected optic nerve that received a cell graft.

Figure 5:

Representative images of a longitudinal section through an optic nerve 14 days after an optic nerve sheath preserving transection and transplantation of neural stem cells (NSCs) expressing the fluorescent protein tdTomato. (A) Immunolabeling for glial fibrillary acidic protein (GFAP) demonstrated complete separation of the transected optic nerve ends. (B) NSCs expressing tdTomato were contained within the space created by the described technique and continued to survive following transplantation. (C) Grafted NSCs were directly apposed to both cut ends of the transected optic nerve. Scale bars, 200 μm. Please click here to view a larger version of this figure.