FIG. 2.

Effect of hemin concentration on rMnP production. Cultures of transformant pssCsMnP1-4 were grown in minimal medium containing 5% maltose. The treatments varied with respect to the timing of hemin addition; 0.25 mg of hemin per ml was added with the inoculum (grey bars) or 24 h after inoculation (solid bars) or 0.5 mg of hemin per ml was added with the inoculum (bars with vertical lines) or 24 h after inoculation (open bars). Controls contained no hemin (stippled bars). Activity was measured at the times indicated.