FIG. 4.

SDS-PAGE of rMnPs. (A) Extracellular fluids from 48-h cultures were concentrated 20-fold, and 0.05 U of MnP activity from transformant pTAAMnP1-3 (rH4) (lane 1) or pssCsMnP1-4 (Cs-rMnP1) (lane 3) was treated with β-mercaptoethanol, boiled, and applied to the gel. After Q-Sepharose chromatography, 0.05 U of rH4 and 0.05 U of Cs-rMnP1 were subjected to the same treatment and applied to the gel (lanes 2 and 4, respectively). (B) Zymogram obtained with o-dianisidine as the substrate, as described in Materials and Methods. Lane 1, 0.05 U of rH4; lane 2, 0.05 U of Cs-rMnP1.