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IEF of rMnPs. Portions (0.03 U) of Cs-rMnP1 (lanes 1 and 3) and rH4 (lanes 2 and 4) were loaded, and after the gel was electrophoresed, the proteins were either stained with Coomassie blue (lanes 1 and 2) or developed for activity with o-dianisidine (lanes 2 and 3).