TABLE 1.

Designed Y2 receptor gene primers used for DNA-methylation.

	Chromosomal location	Distance from the start codon
P1	Chr3:82455447-82455477	p6675–p6678	Forward primer GGAGGAGGATAGGAGATAGAAGT Reverse primer (biotinylated) CAATTCCCCTACCTATATCCTTT Sequencing primer AGATAGAAGTGGAGAGA
P2	Chr3:82455487-82455512	p6713–p6718	Forward primer GTTTGGAAGGAAAGGATATAGGT Reverse primer (biotinylated) AAACACTAATCTCCAATCTCTAACT Sequencing primer ATAGGTAGGGGAATTG
P3	Chr3:82455562-82455584	p6788–p6798-p6803	Forward primer AGGGAGTGAGGTAGATAAGTTT Reverse primer (biotinylated) ACTACCCTACAACTTTTCTCTACA Sequencing primer AGTGTTTTTTAATTTTAGTTGTA
P4	Chr3:82455669-82455701	p6895–P6902	Forward primer GATTGGAGATTAGTGTTTTTTAATTTTAGT Reverse primer (biotinylated) TATTCACATAACCAAAAACCACAACTC Sequencing primer AGTGTAGGGTAGTGG