Table 3.

qPCR cycling conditions

Steps	Temperature	Time	Cycles
Initial Denaturation	95°C	300 s	1
Denaturation	95°C	5 s	40 cycles
Annealing	55°C–68°C	10 s
Extension	72°C	10 s
Melting curve	72 → 90°C	n/a	1
Hold	4°C	∞