Figure 1.

A C. elegans intestinal metabolic regulatory network

(A) Outline of RNAi screen with 19 intestinal reporter strains using the met-2.0 library containing 1,495 metabolic genes. The primary screen was performed in triplicate, and hits were retested with all strains in duplicate. Related to Tables S1 and S2.

(B) Examples of interactions between metabolic genes and intestinal gene promoters. Throughout the paper fluorescence images are shown with bright field images as insets. We used scale bar = 250μm, throughout the study.

(C) Graph outlining potential information flow in combined MRN and GRN.

(D) k refers to degree, or number of interactions. The in-degree (k-in) distribution of the MRN, which is defined as the number of metabolic gene knockdowns that increase or decrease promoter activity in the intestine. Related to Table S3.

(E) The percentage of TFs (top, black) versus metabolic genes (bottom, green) that regulate each intestinal promoter. Data are shown as the percent of RNAi knockdowns causing a change in promoter activity relative to the number of RNAi clones in the TF (top) or metabolic gene (bottom) RNAi library.

(F) Two types of wiring in the combined MRN and GRN.

(G) The out-degree (k-out) distribution of the MRN, which is defined as the number of intestinal gene promoters affected by each metabolic gene knockdown. Related to Table S3.

(H) Detection of each of eight possible feedforward loops involving metabolic genes (M), the TF SBP-1 (S), and downstream promoters (P). The numbers in red are according to numbering by Alon and colleagues (Mangan and Alon, 2003). Numbers and percentages below each circuit indicate total numbers and percentage of possible circuits, i.e., which of the M- > S- > P are confirmed by detection of M- > P in the MRN, between brackets.

(I) Diagram of potential information flow of interactions involving the TF SBP-1.