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. 2022 Jul 1;14:921573. doi: 10.3389/fnagi.2022.921573

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Copyright © 2022 Lopez-Toledo, Silva-Lucero, Herrera-Díaz, García, Arias-Montaño and Cardenas-Aguayo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunodetection of autophagy (SQSTM1 and LC3II), lysosomal (LAMP2 and CatD) and proteasomal (PSMB5) markers in fibroblasts from FAD individuals and control subjects (NA). Proteins were evaluated by Western blotting. (A) Blots for fibroblast protein extracts from control (6) and FAD (6) individuals. Samples were analyzed in duplicate. (B) Analysis of SQSTM1, LC3II/LC3I, LAMP2, CatD and PSMB5. Protein levels were normalized to GADPH in the sample. The points on the graphs represent the average value of duplicates for each sample. Statistical analysis in (B) was performed with Student’s t-test.