TABLE 2.
Antibodies used in immunofluorescence and western blot protocols.
| Antibody | Catalog | Species | Description | Dilution | Company |
| Vimentin | Sc-6260 | Rabbit polyclonal | Mesenchymal marker | 1/250 | Santa Cruz, Dallas, TX, United States |
| S100A4 | GTX134743 | Rabbit polyclonal | Fibroblastic marker | 1/250 | Genetex, Irvine, CA, United States |
| SQSTM1/p62 | BS-2951R-TR | Rabbit polyclonal | Autophagy marker | 1/1000 | Bioss Antibodies Inc., Woburn, MA, United States |
| LC3 | GTX17380 | Rabbit polyclonal | Autophagy marker | 1/1000 | Genetex, Irvine, CA, United States |
| LAMP2 | ABL-93-S | Rat monoclonal | Lysosomal marker | 1/500 | Developmental Studies Hybridoma Bank, Iowa city, IA, United States |
| CatD | GTX62063 | Rabbit polyclonal | Lysosomal marker | 1/1000 | Genetex, Irvine, CA, United States |
| PSMB5 | GTX50128 | Rabbit polyclonal | Proteasome marker | 1/1000 | Genetex, Irvine, CA, United States |
| p-AKT Ser473 | GTX50128 | Rabbit polyclonal | Cell survival marker | 1/1000 | Genetex, Irvine, CA, United States |
| AKT | 2938s | Rabbit polyclonal | Cell survival marker | 1/1000 | Cell Signaling Technology, Danvers, MA, United States |
| p-GSK3A/B | 9327s | Rabbit polyclonal | Kinase marker | 1/1000 | Cell Signaling Technology, Danvers, MA, United States |
| total GSK3A/B | 5676p | Rabbit polyclonal | Kinase marker | 1/1000 | Cell Signaling Technology, Danvers, MA, United States |
| p-ERK1/2 | 4695s | Rabbit polyclonal | Kinase marker | 1/1000 | Cell Signaling Technology, Danvers, MA, United States |
| total ERK1/2 | 9101s | Rabbit polyclonal | Kinase marker | 1/1000 | Cell Signaling Technology, Danvers, MA, United States |
| p-tau Thr231 | MBS9600919 | Rabbit polyclonal | Tau phosphorylation marker | 1/1000 | Biosource, San Diego, CA, United States |
| p-tau Thr181 | GTX50171 | Rabbit polyclonal | Tau phosphorylation marker | 1/1000 | Genetex, Irvine, CA, United States |
| p-tau Ser396/Ser404 (PHF-1) | ab184951 | Rabbit polyclonal | Tau phosphorylation marker | 1/1000 | ABCAM, Cambridge, MA, United States |
| p-tau Ser202/Ser205 (AT8) | MN1020 | Mouse monoclonal | Tau phosphorylation marker | 1/1000 | Thermo Fisher Scientific, Waltham, MA, United States |
| total tau (tau5) | 556319 | Rabbit polyclonal | Tau phosphorylation marker | 1/1000 | BD Pharmingen Inc., San Diego, CA, United States |
| AβPP (6E10) | SIG-39320 | Rabbit polyclonal | Tau phosphorylation marker | 1/1000 | BioLegend, San Diego, CA, United States |
| HSP70 | GTX104126 | Rabbit polyclonal | Stress cellular marker | 1/1000 | Genetex, Irvine, CA, United States |
| HSP60 | MCA-1C7 | Mouse monoclonal | Stress cellular marker | 1/1000 | EnCor Biotechnology, Gainesville, FL, United States |
| GAPDH | GTX627408 | Mouse monoclonal | Load control | 1/1000 | Genetex, Irvine, CA, United States |