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. 2022 Jul 1;14:921573. doi: 10.3389/fnagi.2022.921573

TABLE 2.

Antibodies used in immunofluorescence and western blot protocols.

Antibody Catalog Species Description Dilution Company
Vimentin Sc-6260 Rabbit polyclonal Mesenchymal marker 1/250 Santa Cruz, Dallas, TX, United States
S100A4 GTX134743 Rabbit polyclonal Fibroblastic marker 1/250 Genetex, Irvine, CA, United States
SQSTM1/p62 BS-2951R-TR Rabbit polyclonal Autophagy marker 1/1000 Bioss Antibodies Inc., Woburn, MA, United States
LC3 GTX17380 Rabbit polyclonal Autophagy marker 1/1000 Genetex, Irvine, CA, United States
LAMP2 ABL-93-S Rat monoclonal Lysosomal marker 1/500 Developmental Studies Hybridoma Bank, Iowa city, IA, United States
CatD GTX62063 Rabbit polyclonal Lysosomal marker 1/1000 Genetex, Irvine, CA, United States
PSMB5 GTX50128 Rabbit polyclonal Proteasome marker 1/1000 Genetex, Irvine, CA, United States
p-AKT Ser473 GTX50128 Rabbit polyclonal Cell survival marker 1/1000 Genetex, Irvine, CA, United States
AKT 2938s Rabbit polyclonal Cell survival marker 1/1000 Cell Signaling Technology, Danvers, MA, United States
p-GSK3A/B 9327s Rabbit polyclonal Kinase marker 1/1000 Cell Signaling Technology, Danvers, MA, United States
total GSK3A/B 5676p Rabbit polyclonal Kinase marker 1/1000 Cell Signaling Technology, Danvers, MA, United States
p-ERK1/2 4695s Rabbit polyclonal Kinase marker 1/1000 Cell Signaling Technology, Danvers, MA, United States
total ERK1/2 9101s Rabbit polyclonal Kinase marker 1/1000 Cell Signaling Technology, Danvers, MA, United States
p-tau Thr231 MBS9600919 Rabbit polyclonal Tau phosphorylation marker 1/1000 Biosource, San Diego, CA, United States
p-tau Thr181 GTX50171 Rabbit polyclonal Tau phosphorylation marker 1/1000 Genetex, Irvine, CA, United States
p-tau Ser396/Ser404 (PHF-1) ab184951 Rabbit polyclonal Tau phosphorylation marker 1/1000 ABCAM, Cambridge, MA, United States
p-tau Ser202/Ser205 (AT8) MN1020 Mouse monoclonal Tau phosphorylation marker 1/1000 Thermo Fisher Scientific, Waltham, MA, United States
total tau (tau5) 556319 Rabbit polyclonal Tau phosphorylation marker 1/1000 BD Pharmingen Inc., San Diego, CA, United States
AβPP (6E10) SIG-39320 Rabbit polyclonal Tau phosphorylation marker 1/1000 BioLegend, San Diego, CA, United States
HSP70 GTX104126 Rabbit polyclonal Stress cellular marker 1/1000 Genetex, Irvine, CA, United States
HSP60 MCA-1C7 Mouse monoclonal Stress cellular marker 1/1000 EnCor Biotechnology, Gainesville, FL, United States
GAPDH GTX627408 Mouse monoclonal Load control 1/1000 Genetex, Irvine, CA, United States