TABLE 4.
Spots identified in the 2-DE gels of FAD fibroblasts and control.
| Spot | Protein | pI-pH (experimental) | Mw kDa (experimental) | Average normalized volumes |
Fold change | ANOVA (P) | |
| Controls | FAD | ||||||
| 1 | HSP90 | 5.21 | 91 | 2.432e+007 | 5.138e+007 | 2.1 | 6.536e-004 |
| 2 | HSP70 | 5.22 | 74 | 9.669e+007 | 1.449e+008 | 1.5 | 0.048 |
| 3 | CRTC | 4.43 | 62 | 2.749e+007 | 5.187e+007 | 1.9 | 0.031 |
| 4 | TCPB | 6.28 | 57 | 9.962e+006 | 2.503e+007 | 2.5 | 0.044 |
| 5 | ATPB/PDA6 | 5.28 | 48 | 3.255e+007 | 5.442e+007 | 1.7 | 0.036 |
| 6 | ENOA | 6.19 | 48 | 1.062e+007 | 2.279e+007 | 2.1 | 0.003 |
| 7 | SET | 4.86 | 40 | 3.114e+007 | 5.682e+007 | 1.8 | 0.026 |
| 8 | TPM1 | 4.87 | 33 | 2.722e+007 | 4.812e+007 | 1.8 | 0.032 |
| 9 | ANXA | 5.23 | 31 | 7.722e+007 | 1.065e+008 | 1.4 | 0.021 |
| 10 | ESTD | 6.75 | 31 | 1.011e+007 | 1.891e+007 | 1.9 | 0.023 |
| 11 | CANS | 4.97 | 27 | 5.690e+007 | 1.150e+008 | 2.0 | 0.017 |
| 12 | HSP27 | 6.28 | 26 | 3.624e+006 | 6.479e+006 | 1.8 | 0.008 |
| 13 | ATPQ | 5.22 | 22 | 1.761e+007 | 2.624e+007 | 1.5 | 0.035 |
| 14 | FINC | 5.87 | 16 | 1.947e+006 | 4.673e+00 | 2.4 | 0.002 |
| 15 | CALD | 6.07 | 80 | 9.831e+006 | 1.592e+007 | 1.6 | 0.031 |
Volumes were calculated from the densitometry analysis and normalized to total volume in the corresponding gel using SameSpot v5.1. The statistical comparison was performed with ANOVA.