Table 3.
Molar concentrations, amount of reads (% reads identified), and average read depth (reads) of libraries prepared with 12-cycle and 15-cycle amplification in limited-cycle PCR step. Libraries were quantified by using LabChip® DNA High Sensitivity Assay on LabChip® GX Touch HT
|
|
12-cycle library |
15-cycle library |
||||
|---|---|---|---|---|---|---|
| Sample ID | Molarity (nmol/L) | % reads identified | Average read depth | Molarity (nmol/L) | % reads identified | Average read depth |
|
MW-004
|
12.8 |
2.2 |
5216 |
43.77 |
1.9 |
5882 |
|
MW-005
|
6.5 |
1.5 |
3734 |
45.23 |
1.6 |
5270 |
|
MW-019
|
6.8 |
2.1 |
5222 |
36.17 |
2.9 |
9895 |
|
MW-020
|
8.9 |
2.1 |
6445 |
34.85 |
4.1 |
13850 |
|
MW-026
|
4.1 |
1.1 |
4083 |
46.31 |
1.5 |
5523 |
|
MW-051
|
4.8 |
1.9 |
4106 |
46.67 |
2.1 |
6742 |
|
MW-152
|
5.9 |
2.8 |
4272 |
46.02 |
3.9 |
12295 |
|
MW-236
|
11.4 |
2.3 |
5611 |
47.34 |
2.2 |
7196 |
|
MW-290
|
5.1 |
2.0 |
5718 |
37.73 |
2.1 |
7080 |
| MW-302 | 7.9 | 2.0 | 5611 | 47.60 | 2.5 | 8423 |