Table 4.
Quality metrics for sequencing runs compared to assess library normalization methods. In runs 1-3 libraries were normalized with magnetic beads included in the Nextera® XT library prep kit, while in runs 4-6 individual dilution was applied after quantification with LabChip® DNA High Sensitivity Assay on LabChip® GX Touch HT
| Run | No. of samples | Normalization method | Cluster density (K/mm2) | Clusters PF (%) | % bases≥Q30 | % reads identified ± standard deviation (expected %) | % CV |
|---|---|---|---|---|---|---|---|
| 1 |
24 |
Bead-based |
939 |
94.1 |
93.0 |
4.0 ± 1.3 (4.2) |
32.1 |
| 2 |
24 |
491 |
97.8 |
97.1 |
3.9 ± 1.8 (4.2) |
47.1 |
|
| 3 |
24 |
1062 |
91.5 |
93.8 |
4.1 ± 1.6 (4.2) |
40.0 |
|
| 4 |
24 |
LabChip-based | 864 |
93.6 |
93.4 |
4.0 ± 0.6 (4.2) |
16.1 |
| 5 |
28 |
948 |
93.7 |
96.1 |
3.7 ± 0.7 (3.6) |
19.4 |
|
| 6 | 24 | 551 | 96.0 | 95.7 | 4.1 ± 1.7 (4.2) | 41.7 |