Table 1.
Bacterial strain, phage and plasmids used in this study.
| Strain, phage and plasmid | Relevant traitsa | Source of reference |
|---|---|---|
| Escherichia coli | ||
| SM10λpir | Conjugative donor strain | ZOMANBIOb |
| P. aeruginosa strain | ||
| PAO1r | O-antigen deficient P. aeruginosa | (Shen et al., 2018) |
| PAO1rRL5 | a phage L5-resistant mutant of PAO1r | This study |
| PAO1rΔpilZ PAO1rΔpilZ:: pilZ |
PAO1r mutant with a deletion in the pilZ gene PAO1rΔpilZ complemented with pilZ |
This study This study |
| Phage | ||
| L5 | P. aeruginosa lytic phage | This study |
| Plasmids | ||
| pEXG2 | Allelic exchange vector, GmR | (Rietsch et al., 2005) |
| pEXG2-pilZ | Deletion vector for deletion of pilZ | This study |
| pHB20TGm | Complementation vector, GmR | a gift c |
| pHB20TGm-pilZ | Complementation vector for complementation of pilZ | This study |
a
GmR, gentamicin resistance.
b
ZOMANBIO, Beijing Zoman Biotechnology Co., Ltd.
c
a gift from Shuai Le (Army Medical University, Chongqing).