Table 2.

Primers used in this study.

Primer	Sequence (5′-3′)	Function
pilZ-up-F	TTTCTCGAGCAAGGTCGTGTTGCTCGAAC (XhoI)	Amplification of pilZ upstream
pilZ-up-R	GCATGATCCTGTCTAGCGGCAGGTTCCTGCCAGTCGAATATCAGC	Amplification of pilZ upstream
pilZ-down-F	GACTGGCAGGAACCTGCCGCTAGACAGGATCATGCTGGTCGATTC	Amplification of pilZ downstream
pilZ-down-R	TTTTCTAGACGACATCGCGCACGTATTCC (XbaI)	Amplification of pilZ downstream
pilZ-C-F	TTTTCTAGTATGAGTTTGCCACCCAATC (XbaI)	Amplification of gene pilZ
PilZ-C-R	TTTAAGCTTTTACATCGTGTGGGTCG (HindIII)	Amplification of gene pilZ