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. 2022 May 7;9(20):2200742. doi: 10.1002/advs.202200742

Figure 3.

Figure 3

Abrogation of MDM2 in hepatocytes promotes TG‐VLDL secretion. H‐MDM2‐KO and WT littermates fed HFHC diet were used. Serum levels of A) triglyceride (TG) and B) cholesterol under ad libitum feeding (n = 7–10). C) Serum TG levels after tyloxapol injection in the 16 h fasted mice fed HFHC diet for 1 and 4 months (n = 4–5). @ p < 0.05, WT (1 month) vs KO (1 month); # p < 0.05; WT (4 month) vs KO (4 month) (fold change of TG‐VLDL secretion rate). D) Serum TG levels after oral gavage with olive oil in the mice fed HFHC diet for 3 months (n = 5). E–G) Serum samples collected from the mice fed HFHC diet for 4 months after injection with tyloxapol for 3 h were analyzed. E) Immunoblotting analysis of ApoB100, ApoB48, and ApoA‐1 in the serum. The bar chart is the densitometric quantification of ApoB48 and ApoB100 normalized with ApoA‐1. Representative immunoblot images are shown (n = 4). Distribution of F) TG and G) cholesterol in different densities of lipoproteins in the serum samples (CM: chylomicron; VLDL: very low density lipoprotein; LDL: low density lipoprotein; HDL: high density lipoprotein). All data are presented as mean ± SEM. *p < 0.05 and **p < 0.01 (Two‐tailed independent Student's t‐test).