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. 2022 May 7;9(20):2200742. doi: 10.1002/advs.202200742

Figure 6.

Figure 6

MDM2 regulates TG‐VLDL secretion and ApoB expression independent of p53. Hepatic MDM2‐p53 double knockout (DKO) mice and their WT littermates fed HFHC diet were used. A) Immunoblotting analysis of MDM2, p53, and GAPDH in livers of the mice. B) Random fed serum TG levels in the mice fed HFHC diet for 8 weeks (n = 7–8). C) The mice fed HFHC diet for 8 weeks were injected with tyloxapol. Circulating levels of TG upon tyloxapol injection (n = 5). D) Serum samples were collected from the mice after tyloxapol injection for 3 h, followed by immunoblotting analysis of circulating ApoB100 and ApoB48. The right bar chart is densitometric analysis of circulating ApoB100 and ApoB48 normalized with ApoA‐1 (n = 4). E) mRNA and F) protein expressions of ApoB and Mttp in the livers of mice after HFHC feeding for 16 weeks (n = 7–8). Representative immunoblots are shown. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01 (Welch's t‐test for ApoB100 in panel F); two‐tailed independent Student's t‐test for the remaining data). N.S. (Not significant).