Table 2.
Antibodies for flow and direct single cell staining
| Marker | Tumor subtype/cell type | Vendor | Cat # | Clone | Host | Cell line | |
|---|---|---|---|---|---|---|---|
| 1 | EpCAM | Tumor cells | Biolegend | 324202 | 9C4 | Mouse | BT474 |
| 2 | EGFR | Tumor cells | Abcam | ab30 | EGFR1 | Mouse | MDA‐MB‐231 |
| 3 | HER2 | Tumor cells | BioRad | MCA1788 | ICR55 | Rat | BT474, HCC‐1954 |
| 4 | HER2 | Tumor cells | Biolegend | 324402 | 24D2 | Mouse | BT474 |
| 5 | ER (ESR1) | Tumor cells | CST | 13258S | D6R2W | Rabbit | BT474 |
| 6 | PR (PGR) | Tumor cells | CST | 8757S | D8Q2J | Rabbit | BT474 |
| 7 | MUC1 | Tumor cells | Fitzgerald | 10‐M93A | 10‐M93A | Mouse | MDA‐MB‐231 |
| 8 | CD45 | Immune cells | CST | 13917 | D9M8I | Rabbit | PBMC |
| 9 | IgG Control | N/A | CST | 3900 | DA1E | Rabbit | N/A |
| 10 | IgG Control | N/A | Biolegend | 400501 | RTK2758 | Rat | N/A |
| 11 | IgG Control | N/A | Biolegend | 400102 | MOPC‐21 | Mouse | N/A |
| 12 | IgG Control | N/A | Biolegend | 400302 | MPC‐11 | Mouse | N/A |