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. 2022 Jul 11;63(8):5. doi: 10.1167/iovs.63.8.5

Figure 5.

Figure 5.

TOMM20 and AQP5 are co-expressed molecular markers for the same cluster of cytoplasmic vesicles in the bovine lens cortex. (A-1–C-1) High-magnification confocal images of AQP5 immunolabeling (green) and TOMM20 immunolabeling (red) in the peripheral outer cortex (A), medial outer cortex (B), and outer cortex–inner cortex transitional region (C) of the bovine lens as shown in Figure 3A. Linear (A, open arrowheads) and spheroidal, linear (B, C, closed arrowheads) AQP5-containing cytoplasmic vesicles and TOMM20-containing cytoplasmic vesicles colocalize in the outer cortex of bovine lenses. TOMM20-containing and AQP5-containing cytoplasmic vesicles are frequently apposed to fiber cell nuclei (B, arrow; C, closed arrowhead). (A-2–C-2) Enlarged images of AQP5-containing cytoplasmic vesicles indicated by arrowheads in A-1, B-1, and C-1. (A-3–C-3) Replicate images of A-2, B-2, and C-2 with only TOMM20 immunolabeling and DAPI labeling displayed. Scale bars: 10 µm (A-1, B-1, C-1) and 5 µm (A-2, A-3, B-2, B-3, C-2, C-3).